Technical Assistant position in T Cell Engineering and Molecular Biology

Your Tasks

• Laboratory management, ordering consumables, onboarding new colleagues, coordinating repairs, annual inventory, etc.
• Molecular cloning, including site-directed mutagenesis of plasmids and Golden Gate assembly
• Generation and sequence verification of expression constructs; bacterial transformation, plasmid preparation, and maintenance of the plasmid and glycerol stock collection
• Maintenance of cell lines (e.g., Jurkat and derivatives)
• Transfection and electroporation of cell lines and primary cells; production and titration of lentiviral/retroviral vectors and transduction of target cells
• Isolation of PBMCs from whole blood and leukapheresis material; activation, expansion, and cryopreservation of primary human T cells, including rapid expansion protocols (REP)
• Documentation of work in accordance with genetic engineering (GenTG/S1–S2) and biosafety requirements
• Supervising students and apprentices

Your Profile

• Completed training as a biological, medical, or chemical technical assistant (BTA/MTA/CTA), a bachelor’s degree in a life science subject, or comparable knowledge and experience
• Prerequisites:
  • Solid practical experience in molecular cloning
  • Experience in sterile mammalian cell culture, ideally also primary cell culture
  • Previous experience in an immunology or immunotherapy laboratory is advantageous, e.g., PBMC isolation, REP, Jurkat cell culture, transfection/transduction, FACS, or previous experience with the generation and sequencing of NGS libraries
  • Willingness to work with primary human material (blood and tissue samples)
  • Careful and complete documentation of experiments, ideally using an electronic lab notebook
  • Strong English skills
• Precision, commitment, teamwork, and flexibility regarding tasks and approaches, as well as strong coordination and organizational skills

We Offer

 Excellent framework conditions: state-of-the-art equipment and opportunities for international networking at the highest level

 30 days of vacation per year

 Flexible working hours

 Remuneration according to TV-L incl. occupational pension plan and capital-forming payments

 Possibility of part-time work

 Family-friendly working environment

 Sustainable travel to work: subsidized Germany job ticket

 Unleash your full potential: targeted offers for your personal development to further develop your talents

 Our Corporate Health Management Program offers a holistic approach to your well-being

Are you interested?

Then become part of the DKFZ and join us in contributing to a life without cancer!

​Duration: The position is initially limited to 2 years.

Application Deadline: September 3, 2026

Applications by e-mail cannot be accepted.

Please also note that we cannot return applications submitted by post.