Fixed-term

Work duties and areas of responsibility As a PhD student in the doctoral program visi-on-brain in our research group, you are responsible for designing and producing data, analyzing the results and reporting on scientific projects (in writing and orally) – in collaboration with the research group leader and members of the group. Your duties mainly include establishment, culture, characterization, differentiation and studies of iPSC (induced pluripotent stem cells) derived models of the human brain in 2D and 3D, including downstream characterization of cell identity, cell function and networking. The tasks also include administrative work and collaboration with other disciplines. You will use cell and molecular biology methods, bioinformatics, advanced cell culture, RT-PCR, immunostaining, microscopy, flow cytometry, analysis of single cell and bulk omics sequencing data as well as mathematic modeling. Other existing tasks within the research group may also be relevant, such as involvement in other projects in the research group, administration, conference travel, presentations of research and supervision of students. As a Marie Curie doctoral network PhD student, you will be integrated into visi-on-brain and closely link your project to other projects in this European network and internship at a company. We offer Lund University is a public authority which means that employees get particular benefits, generous annual leave and an advantageous occupational pension scheme. Read more on the University website about being a Lund University employee Work at Lund University Within the MSCA network, we offer: MSCA allowances package (living + mobility; family allowance if applicable), in accordance with programme rules.  Comprehensive international training programme through the Doctoral Network (including courses, summer schools, and transferable skills training). Secondments with consortium partner Joint supervision and access to a multi-stakeholder ecosystem. Qualifications  Requirements for the position are: a second-cycle degree, completed course requirements of at least 240 higher education credits, of which at least 60 higher education credits at the second-cycle level, or in some other way acquired essentially equivalent knowledge within or outside the country. good ability to utilize the doctoral studies The employment requirements are: Completed undergraduate studies in stem cell biology, neurobiology, molecular biology or equivalent Documented experience in culturing human iPSCs Experience of neural differentiation of human iPSC in 2D and 3D Experience in 3D organoid cultures Experience of standardization of methods and cells Experience in calcium signalling and imaging Ability to plan and use methods such as cloning, flow cytometry, immunostaining and imaging, statistical methods, bioinformatics Scientific skills to plan and analyze experiments Skills to function well in collaborative projects both within the group and with other research groups as well as with other disciplines Positive attitude, hard work and a drive to succeed Documented experience of presenting research in speech and writing Great emphasis will be placed on personal qualities. The tasks require that you are structured, accurate, persistent and with great interest in understanding human brain development. Eligibility (MSCA Doctoral Network Rules) Applicants must meet all MSCA DN eligibility criteria at the time of recruitment:  Doctoral Status Rule: The applicant must not already hold a PhD/doctoral degree.  Mobility Rule: The applicant must not haver esided or carried out their main activity (work/studies) in the Sweden for more than 12 months in the 36 months immediately before the recruitment date.  The following experience is of further merit: Tissue engineering work Work with microfluidics Experience with Calcium imaging Development of algorithms Administrative tasks Interdisciplinary collaborations Good ability to supervise students How to Apply The application should include the following: Personal letter (max 1 page) including description of how you fulfill the requirements Short CV Graduation certificate 2 Reference letters About the employment The position is a fixed-term appointment of four years (100%), with a start date no later than 1 December 2026. The main rule is that employment at Lund University is conducted in Sweden, so you are expected to be able to perform your work on site in Lund. Provisions regarding employment as a doctoral student are found in the Higher Education Ordinance (1993:100), Chapter 5, Sections 1-7. Provisions regarding admission to doctoral studies are found in Chapter 6, Section 3, Higher Education Ordinance (1993:100). More information is available on the university’s website. More Information  Full details and official call documentation: https://marie-sklodowska-curie-actions.ec.europa.eu/actions/doctoral-networks Direct link to the project: https://cordis.europa.eu/project/id/101227124 Apply Now

Lund University Lund University was founded in 1666 and is repeatedly ranked among the world’s top universities. The University has around 46 000 students and 8 500 staff based in Lund, Helsingborg and Malmö. We are united in our efforts to understand, explain and improve our world and the human condition. Description of the workplace The Stem Cells, Aging and Neurodegeneration group is using stem cell, reprogramming and genome engineering to study and develop therapies for neurodegenerative disorders and brain tumors. The group is led by Associate professor Henrik Ahlenius and includes two post-docs, two associate researchers and a few master students with different nationalities and professional background. The group is placed at the biomedical center (BMC), Department of Experimental Medical Science and part of several Strategic Research Areas (SRAs) in stem cells (StemTherapy), neurodegeneration (MultiPark) and cancer (LUCC) at LU. Read more about the team HERE. We are looking for an experienced and ambitious doctoral candidate to broaden and strengthen our expertise in advanced cell-based 3D tissue models and modeling of neurodegenerative disorders. As a work place we value an open, inclusive and equal work environment. Project description You will work with the continued development, validation and use of novel reprogramming technologies, 3D tissue models and genome engineered pluripotent stem cell-based models of Alzheimer’s disease. The work includes both development of new models as well as using established models to study the role of glial cells in Alzheimer’s disease. The project is part of the Marie Sklodowska-Curie Doctoral Network VISI-ON-BRAIN which aims to train doctoral candidates in cutting-edge strategies to model brain disorders. The ultimate goal of VISI-ON-BRAIN is to train a new generation of scientists with the skills to advance therapeutic discovery. Read more about VISI-ON-BRAIN here and here. Project Reference: 101227124 Tasks and responsibilities The main task for a doctoral student is to devote yourself to your doctoral studies, therefore you will be both admitted as a student and employed at Lund University. You will be responsible for planning and performing research to further the development and use of genome engineering of pluripotent stem cells as well as reprogramming pluripotent cells to neural and non-neural cells. The results from these studies will be the basis for creating and studying glial cell interactions in state of the art cell-based 3D tissue models of Alzheimer’s disease. The work includes genome engineering of pluripotent stem cells, cellular reprogramming; molecular cloning, vector design,  omics and bioinformatics as well as cellular, molecular and functional assays mainly in vitro. The main task is laboratory and analysis work. In addition, presenting your research nationally and internationally, writing research articles as well as grant applications is part of the job description. Some supervision and teaching might be included. You will be part of two vibrant strategic research areas and have access to several career development activities. What we offer Lund University is a government agency, which means that you get special benefits, generous holidays and a favourable occupational pension.  As a doctoral student at Lund University, you are both admitted as a student and employed, which gives you several benefits. Read more about being admitted and employed  at the university’s website. Lund University has generous support for parental leave and favourable offers for training and wellness. Find out more on the University website about working with us Work for us As part of VISI-ON-BRAIN you will be entitled to the MSCA allowances package (living + mobility; family allowance if applicable), in accordance with programme rules Qualifications Requirements for the position are: a second-cycle degree, completed course requirements of at least 240 higher education credits, of which at least 60 higher education credits at the second-cycle level, or in some other way acquired essentially equivalent knowledge within or outside the country. good ability to utilize the doctoral studies The employment requirements are: Must not already hold a PhD/doctoral degree MSCA mobility rules apply: The applicant must not have resided or carried out their main activity (work/studies) in Sweden for more than 12 months in the 36 months immediately before the recruitment date.  Second cycle education within cellular and molecular biology, neurobiology, biotechnology or similar Positive attitude, hard working and a drive to succeed Strong background in cell and molecular biology Excellent team player Good at taking own initiatives Excellent communication and collaborative skills Good command of the English language Experience working with pluripotent stem cells Merits for the position are: Experience from genome engineering and inducible systems Experience from cellular reprogramming towards neural linages Experience with cellular, tissue and disease models Experience with fluorescence and confocal microscopy, imaging and analysis using imaging software Experienced with microfluidics and/or on-chip solutions Experience from neural 3D cultures such as organoids/spheroids Great emphasis will be placed on how the applicant, through his/her experience and competence, is judged to have the ability needed to complete the doctoral studies About the employment The position is a fixed-term, full-time appointment (100%) for a total of four years, with a start date no earlier than 1 September 2026 and no later than 1 December 2026. Rules and regulations concerning employment as a doctoral student can be found in the Higher Education Ordinance (1993:100), Chapter 5, Sections 1-7. Provisions regarding admission to doctoral studies are found in Chapter 6, Section 3 of the Higher Education Ordinance (1993:100). More information is available on the university’s website How to Apply The application should include the following: Personal letter (max 1 page) including description of how you fulfill the requirements Short CV Graduation certificate 2 Reference letters Apply Now

Lund University Lund University was founded in 1666 and is repeatedly ranked among the world’s top universities. The University has around 46 000 students and 8 500 staff based in Lund, Helsingborg and Malmö. We are united in our efforts to understand, explain and improve our world and the human condition. Description of the workplace The research at the division for Biophysical Chemistry is dedicated to the study of biomolecular interactions, structure and dynamics, with the aim of understanding biological systems and processes at the molecular level. The emphasis is on experimental and theoretical studies of the physical properties of proteins in solution. The Center for Molecular Protein Science (CMPS), Department of Chemistry brings together scientists active within the fields of biochemistry, molecular biophysics, structural biology, and physical and theoretical chemistry. Collectively we are around 70 people distributed in 15 research groups. You will join the research group of Kristine Steen Jensen at CMPS, Biophysical Chemistry. Being a doctoral student As a doctoral student, you are both admitted as a student and employed at Lund University. As a doctoral student, you will be trained in a scientific approach. In short, you will be trained to think critically and analytically, to solve problems independently using the right methods, and to develop an awareness of research ethics. In addition, you will have the opportunity to work on projects, to develop your leadership and pedagogical skills. Throughout your studies, you will be guided by supervisors. Doctoral studies end with a thesis and a doctoral degree. More about being a doctoral student at LTH on lth.se. https://www.lth.se/english/study-at-lth/phd-studies/ Subject and project description This PhD project invites you to explore how designed proteins can become building blocks for the materials of the future. The project will focus on the experimental characterization of two-dimensional self-assemblies of AI-designed proteins. These protein architectures are envisioned as programmable nanoscale platforms for sustainable biofabrication and semiconductor-related applications.Using cryo-electron microscopy you will visualize whether the designed proteins assemble into the intended ordered 2D structures. A central part of the project will be to develop spectroscopic assays to follow assembly formation, probe stability, and detect interactions between protein components. These assays will be adapted for high-throughput screening, enabling many protein designs and assembly conditions to be evaluated efficiently.You will also explore how non-native metal-binding groups can be introduced into proteins through genetic code expansion strategies to place metals in specific patterns on a protein scaffold. Together this will open opportunities to tune protein materials for interactions with inorganic and semiconductor components.By connecting molecular design with advanced imaging, spectroscopy, and screening, the project aims to create new routes toward sustainable nanotechnology.The PhD project is part of an interdisciplinary collaboration between de novo protein designers, biophysical chemists, biochemists and semiconductor physicists. Work duties You will primarily devote yourself to your doctoral programme, which includes participation in research projects as well as third cycle courses, seminars and conferences. You will be responsible for production and experimental characterization of self-assembling de novo designed proteins, but will also be involved in selection of promising constructs and are expected to contribute to the overall design strategy. You will work in close collaboration with computational protein designers to establish a proficient feedback loop between experimental results and design strategy. The work duties include: Filtering and selection of de novo designed proteins for characterization Protein expression and purification Design and production of novel protein assemblies with non-canonical amino acids with metal binding properties Developments of high-through-put screening assays Spectroscopic characterization of 2D protein self-assembly Cryogenic electron microscopy of protein-metal assemblies The duties also include participation in teaching and other departmental work (however, a maximum of 20% of working hours). The division is responsible for courses in physical chemistry, NMR spectroscopy and biophysical chemistry. Qualifications To be eligible for admission and employment as a doctoral student, you must fulfil the requirements below. Admission requirements A person meets the general admission requirements for third-cycle courses and study programmes if the applicant: has been awarded a second-cycle qualification, or has satisfied the requirements for courses comprising at least 240 credits of which at least 60 credits were awarded in the second cycle, or has acquired substantially equivalent knowledge in some other way in Sweden or abroad. A person meets the specific admission requirements for third cycle studies in Biophysical Chemistry if the applicant has: a MSc in Engineering, a one-year Master’s degree or other comparable degree with significant components of mathematics,molecular sciences and life sciences. Depending on the student’sresearch project, second-cycle courses in physical chemistry,biophysical chemistry, protein science and/or molecularspectroscopy may be required. Finally, the student must be judged to have the potential to complete the program. Additional requirements: Experience of experimental protein characterization and biophysical measurements with quantitative data analysis. Molecular understanding of protein structure and interactions. Demonstrated ability to work independently. Scientific curiosity Good molecular understanding The applicant should also have good analytical skills (problem solving), be initiative-taking, demonstrate ability to cooperate, be persistent. Good ability to work independently and to formulate and tackle research problems. Good written and oral communication skills Very good oral and written proficiency in English. Other qualifications For the doctoral programme in question, the following are considered as other qualifications: Experience of producing proteins recombinantly is considered a merit. Experience of characterization of the structure of proteins with cryo electron microscopy is considered a merit. Experiences with experimental characterization and quantitative data analysis of protein self-assembly is considered a merit. We offer Lund University is a public authority which means that employees get particular benefits, generous annual leave and an advantageous occupational pension scheme. More about working at Lund University on lu.se. https://www.lunduniversity.lu.se/about-university/work-lund-university About the employment The employment is a fixed-term employment at full time, starting 2026-09-01 or as agreed. Third cycle studies at LTH consist of full-time studies for 4 years. In the case of teaching and other departmental duties, the employment is extended accordingly. Doctoral studentships are regulated in the Higher Education Ordinance (1993:100), chapter 5, 1-7 §§. More about terms of employment for doctoral students on Lund University’s Staffpages. https://www.staff.lu.se/research-and-education/research-support/doctoral-education/terms-employment-doctoral-students  How to

The Department of Biology is a top-tier research and educational facility at the Faculty of Medicine, providing both theoretical and practical instruction in medical biology for Czech and international students. The institute also coordinates the international Ph.D. program in Biomedical Sciences and serves as a prominent international center for excellence in biomedical research. Lumír Krejčí Research Group focuses on the mechanisms of homologous recombination and its role in maintaining genome stability, specifically in repairing fatal DNA damage and ensuring accurate replication. Using interdisciplinary methods, they investigate how defects in these processes lead to the development of cancer, infertility, or genetic diseases. You can also learn more about us on our website, Facebook or Instagram. The workplace: Brno, the Czech Republic The scope of your work will be: Independent research and development activities, including the publication and presentation of research results Share in the organizational provision of research and pedagogical activities, incl. related administration Participation in theoretical and practical teaching at the workplace Preparation of the study and teaching materials Membership in commissions for state final examinations Participation in the education of students in doctoral study programs Supervising undergraduate student theses Required qualification: University degree (Master’s or equivalent) in Molecular Biology, Biochemistry, or a related field obtained abroad. Ph.D. in Molecular Biology or Biochemistry Relevant publication record (supported by an overview of scientific and publishing activities). Proven research experience focusing on genome stability, DNA repair, and DNA replication. Experience working on national and international research projects. Teaching experience Several years of experience with relevant biochemical methods (purification of recombinant proteins, preparation of DNA substrates, enzymatic reactions; experience with phase separation studies is an advantage). International work or study experience (minimum of 2 years) Ability to actively participate in international conferences and professional seminars. Active proficiency in English. Excellent command of the Czech language, both written and spoken. Experience with scientific data analysis and basic programming skills. Proactive approach, initiative, and a willingness for further professional development. Integrity and a clean criminal record (moral and civic standing). We offer: Work in an inspiring intellectual environment Involvement in teaching in a unique simulation centre Professional growth and the possibility of continuous education employee benefits (8 weeks holiday, supplementary pension insurance, free vaccination against tick-borne encephalitis and more) How to apply: Send your application by the above-written deadline – please use the link in the Electronic Application section (see below). Your application must include the following documents: a structured CV, an overview of scientific and publishing activities, photocopies of qualification papers and diplomas, contact information of at least two references (name, position, telephone number). After successfully sending your application, you will receive an automatic confirmation by e-mail. You can provide more information from Mgr. Gabriela Pavlíková by e-mail: pavlikova@med.muni.cz