Designer photoenzymes for selective C-H functionalisations

Details

A PhD studentship is available in the groups of Dr Florence Hardy and Prof Anthony Green, University of Manchester, as part of the cross-institutional BioAID Doctoral Training Programme, including world-leading experts from Queen’s University Belfast, University of Manchester, University of Edinburgh and University of Bristol.

BioAID will train the next generation of scientists in Artificial Intelligence and data-driven approaches for translational biocatalysis, addressing critical needs in the development of sustainable biotechnologies. The programme will equip PhD students with advanced expertise in enzyme science, machine learning, enzyme engineering and synthetic biology, accelerating innovation across sections including pharmaceuticals, agri-tech and clean manufacturing. Students will undertake interdisciplinary, co-supervised projects across biocatalysis and AI, supported by national computing infrastructure, hands-on laboratory training, and strong academic/industry partnerships through co-designed projects and placements. In addition, students will benefit from structured cohort training and tailored professional development delivered by partner institutions.

Project Summary:

This studentship will focus on designing photoenzymes for enantioselective C-H functionalization chemistry using the latest deep learning tools for protein design. Non-selective photo-chemical methods for C-H heteroarylation have been established using di-aryl ketones as a photosensitiser; however, enantioselective versions of these processes remain elusive. Inspired by these studies, we will design photoenzymes to catalyse the 4-pyridination of benzylic Csp3–H bonds. To achieve this goal, we will use advanced computational enzyme design techniques to develop custom proteins that accommodate genetically incorporated photocatalytic moieties and substrate binding pockets to create ideal chiral environments for promoting enantioselective C-C bond forming chemistry.

Through this project, the student will receive training across a range of cutting-edge disciplines and gain expertise in the latest tools for enzyme design and protein structure prediction, organic synthesis, biocatalysis, and directed evolution. The successful candidate will join a growing team of researchers, housed within the Manchester Institute of Biotechnology (MIB) at the University of Manchester. Enquiries can be directed to the supervisors.

Eligibility

The scheme is open to UK students.

· Fulfil The University of Manchester entry requirements 

· Hold (or expect to achieve) a First Class or 2:1 UK honours degree (or international equivalent to be checked with UoM admission team).

· Ideally hold a master’s-level qualification at merit or distinction (or international equivalent to be checked with UoM admission team).

· Demonstrate willingness to travel to partner institutions to complete the programme.

· Fulfil the Faculty of Science and Engineering Postgraduate Researcher person specification

Before you apply

We strongly recommend that you contact the supervisors for this project before you apply florence.hardy@manchester.ac.uk

How to apply

To be considered for this project you’ll need apply here:

In your application, include the project title Designer photoenzymes for selective C-H functionalisations, supervisor Dr Florence Hardy. Select the PhD Chemistry in the programme detail section and include contact details of two referees.

Your application will not be processed without all of the required documents submitted at the time of application, and we cannot accept responsibility for late or missed deadlines. Incomplete applications will not be considered. 

If you have any queries regarding making an application please contact our admissions team FSE.doctoralacademy.admissions@manchester.ac.uk

Equality, diversity and inclusion

Equality, diversity and inclusion are fundamental to the success of The University of Manchester, and are at the heart of all of our activities. We know that diversity strengthens our research community, leading to enhanced research creativity, productivity and quality, and societal and economic impact. 

We actively encourage applicants from diverse career paths and backgrounds and from all sections of the community, regardless of age, disability, ethnicity, gender, gender expression, sexual orientation and transgender status. 

We also support applications from those returning from a career break or other roles. We consider offering flexible study arrangements (including part-time: 50%, 60% or 80%, depending on the project/funder).

Funding Notes

This 4-year PhD project is fully funded and home students, and EU students with settled status, are eligible to apply. The successful candidate will receive an annual tax-free stipend set at the UKRI rate (£21,805 for 2026/27) and tuition fees will be paid. We expect the stipend to increase each year. The start date is October 2026.

We recommend that you apply early as the advert may be removed before the deadline.