Fixed-term

DescriptionWe are seeking a postdoctoral researcher to join a project aimed at understanding how tumorigenesis affects energy expenditure and allocation at the whole-organism level in vivo. This project lies at the interface of developmental biology, metabolism, biophysics, and cancer biology. Our laboratory studies the fundamental processes that govern cellular and organismal energetics and their impact on animal physiology. We have recently developed quantitative approaches to measure energy allocation at the whole-embryo level and its evolution as a function of ploidy during development (Cadart et al., Current Biology, 2023). This project is part of a new collaboration with Professor Goldwasser’s team (Cochin Hospital), which specializes in the study of cancer metabolism in a clinical context (Boudou-Rouquette et al., EBioMedicine, 2021; Tiako et al., Clinical Nutrition ESPEN, 2024). The aim of this project is to establish Xenopus tadpoles as a quantitative whole-organism model to analyze the impact of tumor growth on energy production, consumption, and allocation. The project relies on the development and integration of original experimental approaches, including the manipulation of Xenopus embryos, in vivo imaging of cellular metabolism using FLIM, direct calorimetry measurements, and quantitative analyses linked to clinical metabolic data. The project involves the development of new experimental strategies. It is intended for a candidate who demonstrates autonomy, scientific rigor, and an interest in interdisciplinary approaches. The work environment is interactive and collaborative, with close links to clinical research. Main Activities: Actively contribute to the development of the project’s conceptual framework and hypotheses, based on literature review and scientific exchanges with the two partner teams. Define and implement a coherent set of biological techniques, imaging, and quantitative analyses necessary for the project’s completion. Set up, optimize, and conduct experiments (handling of Xenopus embryos, in vivo metabolic imaging, calorimetric measurements). Process, analyze, interpret, and validate experimental data. Ensure the dissemination, promotion, and communication of the project’s scientific results. Contribute to regulatory processes related to animal experimentation and the use of GMOs. Participate in the preparation of applications for postdoctoral and/or supplementary funding. Contribute to the writing and publication of scientific articles based on the project results. Environment: The postdoctoral researcher will join a young and dynamic team engaged in ambitious interdisciplinary research. The work environment fosters scientific exchange, collaboration with a clinical team, autonomy, and creativity within an inclusive and stimulating framework. Profile Cancer Biology Animal Experimentation (Level II training preferred) Classical Cell and Molecular Biology Techniques Live Imaging National Institute of Health and Medical Research 4 Quantitative Data Analysis and Processing Skills Ability to analyze scientific literature and formulate original research hypotheses Ability to develop, optimize, and adapt new experimental protocols Ability to design and conduct experiments independently Development of scientific and technological expertise in an interdisciplinary context Writing of scientific documents (articles, reports, grant applications) Excellent written and oral communication skills in English Aptitudes: Analytical reasoning and critical thinking Organizational and planning skills Enjoyment of collaborative work and scientific interaction Autonomy, scientific curiosity, and perseverance in exploratory projects Required Experience: You hold a PhD in a relevant field (cancer biology, metabolism, developmental biology). Degree: PhD

Description Inserm is the only French public organization entirely dedicated to biological, medical, and population health research. It has research laboratories throughout the country, organized into 12 Regional Delegations. Our institute brings together 15,000 researchers, engineers, technicians, and administrative staff, all with a common goal: to improve everyone’s health through advances in knowledge about living organisms and diseases, innovation in treatments, and public health research. Joining Inserm means becoming part of an institute committed to gender equality and professional equality, diversity, and supporting its employees with disabilities, from recruitment and throughout their careers. To promote well-being at work, Inserm pursues an active policy regarding working conditions, based in particular on a fair work-life balance. In 2016, Inserm received the European HR Excellence in Research label and has committed to evolving its recruitment and evaluation practices for researchers. The Position: We are recruiting an experienced postdoctoral researcher (2-3 years post-PhD) to study the cellular and molecular determinants of impaired epithelial regeneration and barrier dysfunction in chronic inflammatory bowel diseases (IBD: Crohn’s disease, ulcerative colitis) and metabolic disorders, with a particular focus on obesity. The central objective is to develop and validate a microphysiological human gut-on-a-chip system, derived from patient material and environmentally controlled, enabling direct functional interrogation of human tissues. Beyond modeling a pathophysiology relevant to the disease, the platform will enable quantitative and patient-specific phenotyping of epithelial alterations (e.g., stem/progenitor cell dynamics, differentiation trajectories, junctional integrity, permeability, wound closure/repair) and will support functional stratification for personalized medicine. Ultimately, the gut-on-a-chip will provide an ex vivo framework for (i) characterizing patient-specific intestinal functional alterations and (ii) evaluating individual responses to drug candidates, in order to prioritize personalized therapeutic strategies aimed at restoring epithelial integrity and promoting mucosal healing. The work integrates complementary human approaches, including patient-derived intestinal and colorectal organoids, our patented gut-on-a-chip microfluidic platform, functional genome editing, and high-content 3D imaging. Research project: We invite applications for an experienced Postdoctoral Fellow (2–3 years post-PhD) to investigate the cellular and molecular determinants of impaired epithelial regeneration and barrier dysfunction in inflammatory bowel diseases (IBD; Crohn’s disease, ulcerative colitis) and metabolic disorders, with a specific focus on obesity.The central objective is to engineer and validate an environment-controlled, patient-derived intestine-on-chip (gut-on-chip) microphysiological system enabling direct functional interrogation of human patient material. Beyond modeling disease-relevant physiology, the platform will deliver quantitative, patient-specific phenotyping of epithelial alterations (eg, stem/progenitor dynamics, differentiation trajectories, junctional integrity, permeability, wound closure/repair) and support functional stratification for personalized medicine. Ultimately, the intestine-on-chip will serve as an ex vivo framework to (i) characterize individual patient alterations and (ii) evaluate patient-specific responses to candidate interventions, enabling priority of personalized therapeutic strategies aimed at restoring epithelial integrity and promoting mucosal healing.The work integrates complementary human-relevant approaches, including patient-derived intestinal and colorectal organoids, our in-house microfluidic intestine-on-chip platform, functional genome editing, and high-content 3D imaging.The successful candidate will combine quantitative phenotyping with mechanistic disturbance to resolve the cellular events and signaling networks underlying defective regeneration and barrier failure in IBD and obesity. Expected outcomes include mechanistically grounded biomarkers and actionable targets, as well as a validated experimental pipeline for patient-centric testing of barrier-restoring and pro-regenerative strategies. Fixed-term contract of 12 months, renewable twice, full-time (38.5 hours per week). Host institution: Institute of Metabolic and Cardiovascular Diseases – U1297 – Occitanie Pyrénées Regional Delegation, 1 avenue Jean Poulhès – 31432 Toulouse Cedex 4. Unit Director: Dominique Langin. Reporting CSS (Centre de Soins Sociales – Social Security Centre): 3. Team Leader: Anne Bouloumie ProfileWork on an integrated experimental program investigating barrier dysfunction and impaired regeneration in IBD and obesity: study design, hypothesis prioritization, implementation, interpretation, and reporting. Establish, culture, and maintain human intestinal and colorectal organoids and fibroblasts derived from patients (healthy controls, IBD, obese patients), ensuring rigorous quality control, traceability, and reproducibility (donors, cell lines, passages, lots/batches). Develop and implement quantitative functional assays of epithelial regeneration and barrier function (e.g., wound healing, proliferation/differentiation dynamics, cell adhesion, permeability, and/or TEER when applicable), with appropriate hierarchical experimental designs (donor/cell line/batch). Perform functional genetic perturbations to investigate candidate pathways (CRISPR/Cas9 editing; CRISPRi/a as appropriate; gene delivery via viral and non-viral systems), including construct design, validation, and phenotypic readouts. Integrate organoid and fibroblast models into the gut-on-a-chip platform, contributing to the setup and operation of the microphysiological system (seeding strategies, flow/shear and environmental control, sampling workflows), in close collaboration with a PhD student and engineering partners, and adapting readouts for patient-specific phenotyping and intervention testing. Acquire and analyze high-content 3D imaging data (confocal and light-sheet microscopy), including sample preparation/clarification as needed, quantitative image analysis, and the development/maintenance of robust pipelines for structural and functional metrics. Conduct multi-level molecular and cellular profiling of tissues and organoids (histology/immunostaining, transcriptomic/protein analyses, and single-cell approaches depending on implementation) and integrate these profiles with functional phenotypes. Collaborate within the PEPR MED-OOC/ENVie consortium to integrate imaging, functional, and multi-omics data for patient stratification and mechanistic inference. Ensure rigorous documentation and reproducibility (SOPs, electronic lab notebooks, metadata, QC reports, data management/curation). Present results at internal meetings and international conferences. Contribute substantially to the preparation of manuscripts and, where applicable, to project/funding deliverables. Apply high standards of good scientific practice, biosafety, and compliance with institutional quality procedures for human samples (traceability, consent/ethical constraints, data protection where applicable). Mentor and support junior researchers/students as needed (e.g., PhD students, Master’s students). Expertise: Advanced culture of intestinal/colorectal organoids and quality control: traceability, batch management, reproducibility, standardization. Development and implementation of functional barrier/regeneration assays: permeability, tight junctions, repair/healing; TEER (Team Energy Evaluation) is an asset where applicable. Robust experimental designs adapted to nested/hierarchical data (donor/line/batch) and management of batch effects. Molecular and cellular biology: immunostaining, qPCR, protein analyses; interest/experience with multi-level approaches (multi-omics/single-cell as needed). Genome editing and functional genetics: CRISPR/Cas9, CRISPRi/a as appropriate; viral and non-viral gene delivery; associated phenotypic readouts. High-resolution 3D imaging (confocal, light-sheet) and quantitative image analysis workflows (standard tools; scripts preferred). Interest in organ-on-a-chip/microfluidics and the ability to integrate readouts into MPS systems in collaboration with engineers and PhD students. Documentation and reproducibility: lab notebook,

DescriptionOur laboratory has one position available for postdoctoral fellow in the area of Cancer Biology and Microenvironment. We are interested in mechanisms of tumor initiation and resistance, with an emphasis on tissue long lived cells such as stromal cells, and their crosstalk with tumor cells, immune cells and the vasculature. We perform mostly in vivo studies, combining lineage tracing and depletion models, flow cytometry, confocal microscopy and unbiased approaches such as single cells transcriptomics, spatial transcriptomics and epigenetics to characterize molecular and cellular mechanisms. Using these approaches, we previously identified an essential role for specific subsets of stromal cells in tissue repair and antitumor immunity (Dulauroy et al., Nature Medicine 2012; Stzepourginski et al., PNAS 2017; Jacob et al., Cell Stem Cell 2022; Di Carlo et al., Nature Immunology 2023). Building on these findings, we aim at identifying novel mechanisms regulating the tumor microenvironment and antitumor immunity, with a focus on solid tumors such as breast cancer and lung cancer. ProfilRecent PhD degree (including in late 2025) in tumor biology, vascular biology, immunology or related biomedical field, with at least one accepted publication and lab experience in one or more areas that include: breast cancer, lung cancer, antitumor immunity, vascular biology, stromal/mesenchymal cells. Highly motivated candidates with interest in areas of research at the intersection of tumor biology, the stromal microenvironment and immunology are strongly encouraged to apply. Specific requirements: Required: Solid experience with in vivo cancer murine models (breast cancer and/or lung cancer) and flow cytometry Desirable: Previous experience in transcriptomic analysis (scRNAseq), scripting languages (R) and Seurat. Would be a plus: Previous experience in epigenetic analysis Informations complémentairesFor more information and to apply, send a motivation letter, CV and contact information for 2 references to Lucie Peduto (lucie.peduto@pasteur.fr).

DescriptionFor the UMR 1249 animal facility at the transgenic mouse breeding site, the incumbent will be responsible for the care of the animals, equipment, and premises. They will monitor all breeding and maintenance processes for animal lines. They will perform technical tasks as needed by site users. They will be a point of contact for users in the area and will report to the head of the animal facility. Main activities: They will be responsible for the care of the animals, equipment, and premises. They will implement and monitor breeding programs according to the experimental needs of the research teams. They will monitor all processes for maintaining experimental lines (mating, births, weaning, sexing, animal identification). They will perform experimental procedures in accordance with the researchers (force-feeding, injections, vaginal plugs, weighing, etc.). They will collect data related to livestock monitoring and ensure its entry into the Expeboard management software. They will participate in the implementation of sentinel animal programs, monitor animal health, and administer necessary treatments. They will be responsible for basic maintenance of the facilities. They will monitor environmental parameters and ensure the proper functioning of equipment. They will inform their supervisor of any malfunctions within the department. They will perform euthanasia in accordance with ethical guidelines. They will dispose of waste while adhering to hygiene and safety regulations. They will implement and enforce the platform’s internal regulations and those related to animal husbandry. They will monitor stock levels of consumables (feed/bedding, cleaning and hygiene products, environmental enrichment, etc.). They will record their activities, observations, and malfunctions daily in a logbook. Profile Knowledge of methods for managing genetically modified rodent colonies and breeding techniques. General knowledge of animal biology (physiology, anatomy, behavior, reproduction, animal behavior, etc.) and mouse genetics. Knowledge of regulations relating to animal experimentation. Knowledge of hygiene and safety rules related to working in animal facilities. Skills: Putting regulations into practice (hygiene and safety, animal experimentation, etc.). Mastering a set of animal breeding techniques. Administration of substances, biological sampling. Determining, based on symptoms, an abnormality or suffering and knowing how to react. Handling an animal (grabbing, restraint, tranquilizing) Skills: Teamwork and initiative , dynamism, curiosity, rigor, autonomy , organization, and meticulousness Job specifics/constraints: Animal handling, work in confined spaces, lifting of loads Desired experience: Experience working in a rodent pet shop, operator-level experimental authorization, autoclave certification