Fixed-term

Investigating the role of P2X7 receptor intracellular domains in cell signalling

Project Description P2X7 receptors are ion channels that are found on the surface of immune cells, where they respond to the binding of extracellular ATP, a damage signal released by dying cells in infection and injury. ATP binding to P2X7 receptors leads to calcium influx and initiates a downstream signalling cascade, resulting in the formation of large pores in the plasma membrane, changes in cell shape, protein secretion, kinase activation and changes in gene expression. Both ion channel function and downstream signalling are highly regulated by the 30 amino-acid N-terminal and 240 amino-acid C-terminal intracellular domains of the receptor (NTD and CTD), at least in part via interaction with lipids in the plasma membrane, but we know little about their interactions with other biomolecules, or which parts of them are involved in signalling pathways. The cryoEM structure of full-length rat P2X7 shows that both the NTD and the cysteine-rich N-terminal portion of the CTD (C-Cys anchor) are palmitoylated, anchoring these domains to the inner leaflet of the plasma membrane, and that the C-terminal portion of the CTD (ballast) forms a globular domain containing binding sites for dinuclear zinc and GDP/GTP, raising intriguing questions about its potential function. Recent work suggests that the ballast domain can bind to calcium-calmodulin, and that this may affect its conformation. The focus of this project is to investigate the role of P2X7 intracellular domains in downstream signalling, using a combination of molecular modelling, site-directed mutagenesis, protein expression, protein purification, functional assays and direct structural studies. Particular focus will be given to understanding the role the ballast domain in coupling to intracellular signalling cascades. How to apply:  You can apply online – consideration is automatic on applying for a PhD in Biosciences Please use our online application service at https://www.cardiff.ac.uk/study/postgraduate/research/programmes/programme/biosciences-phd-mphil-md Please specify that you are applying for this particular project, the supervisor and source of funding. Information on the application process can be found here http://www.cardiff.ac.uk/study/postgraduate/applying Academic criteria: Applicants should have obtained, or be about to obtain, a first or upper second-class UK honours degree, or the equivalent qualification gained outside the UK, Applicants with a lower second class will only be considered if they also have a Master’s degree. Please check the entry requirements of the home institution for each project of interest before completing an application. Academic qualifications are considered alongside significant relevant non-academic experience. English requirements: If English is not your first language you will need to meet the English language requirements of the university that will host your PhD by the start of the programme. Please refer to the relevant university website for further information. Funding Notes This project is suitable for self funded or externally funded students looking for a PhD position at Cardiff University. Funding required will need to cover the tuition fees, bench fees and living costs. As a guide here are the fees for the 2024/25 period (Per annum) International Tuition fees – £25,450 Home Tuition fees- £4,786 There is a guide to living expenses here View Website Apply Now

Position of Animal Attendant in a DBT funded project entitled “Virulence of C. albicans GPI biosynthetic mutants exhibiting hyperfilamentous phenotypes- a study using mammalian cell lines and mouse models”

School of Life Sciences Jawaharlal Nehru University New Delhi – 110 067 Applications are invited for the following post on purely temporary basis in a DBT funded project entitled “Virulence of C. albicans GPI biosynthetic mutants exhibiting hyperfilamentous phenotypes- a study using mammalian cell lines and mouse models” (BT/PR51192/MED/29/1662/2023)  in the laboratory of undersigned. The project duration is till the end of the project. The desired qualifications and emoluments are as follows: 1.    Post: Animal Attendant one Essential qualifications: Graduate degree in any discipline Desirable qualifications: Prior experience in immunology / animal tissue culture lab and small animal care and maintenance will be preferred. Emoluments: As per the norms of the funding agency [17,494/-P.M. consolidated] Upper Age Limit (years): 50 Tenure: The position is purely temporary, and may continue until mid 2027 (the end of project) based on satisfactory performance. Application on a plain paper along with CV, all self-attested certificates, experience and/or training certificate and one reference letter should reach the undersigned on or before April 23, 2026. Applications sent through email will not be considered. Interview will be conducted online / offline. Shortlisted candidates will be called for interview by email. Please note there will be NO TA/DA for attending the interview. Dr. Niti Puri,  Assistant Professor, Cellular and Molecular Immunology Laboratory, Rooms 322/313, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India Phone: 91-11-26738742 (Office) e mail: purin[at]mail[dot]jnu[dot]ac[dot]in

Position of Postdoctoral Research Fellow in cardiac biology

The position A Postdoctoral Research Fellow position is available at the Department of Medical Biology, with the project “Infective endocarditis – investigating early biomarkers, imaging and cellular mechanisms in cardiac valves in mouse a model”. The project is run by Brage Håheim and associated with the Cardiovascular Research Group at the Faculty of Health Sciences. The position is a fixed term position for a duration of 3 years. It is a prerequisite that the applicant can carry out the project over the full course of the employment period. No person may hold more than one fixed term position as a Postdoctoral Research Fellow at the same institution. As a Postdoctoral Research Fellow, you will have the opportunity to advance your scientific career and further qualify for academic positions. If you do not already have the educational competence that meets the requirements for an Associate Professor position in Norway, UiT will arrange such competence during the employment period. The workplace is at UiT in Tromsø. You must be able to start in the position within a reasonable time after receiving the offer. Unless special circumstances indicate otherwise, postdoctoral projects shall be planned with stays abroad. The duration should be at least 6 months. The project/position’s field of research The project is run by Associate Professor Brage Håheim, in collaboration with tenure staff of the Cardiovascular Research Group. The cardiovascular research group is specialized in the study of cardiovascular pathophysiology and Dr Håheim is focused on infective endocarditis and hemodynamics. Specifically, this project focus on improving the outcome of infective endocarditis (IE) by improved pathophysiological understanding, diagnostics and treatment. Infectious endocarditis (IE) is an infection of intracardiac structures. Globally, the annual incidence of IE is 2-10/100,000.  The mortality rate for IE remains high, ranging from 15-30%. Early and accurate diagnosis is critical, and treatment primarily consists of prolonged intravenous antibiotic therapy tailored to the causative organism. In severe cases, surgical intervention may be necessary to repair or replace damaged heart valves or remove infected material. IE exist in the crossroad of cardiovascular, microbiological, immunological and medical imaging research. It is a difficult disease to study and dependent on in vivo models due to the importance of microbe-host interaction. We therefor propose a project to establish an in vivo experimental animal model to study IE. Our plan is to injure the aortic valves in live mice and intravenously inject staphylococcus aureus three days later. Within seven days, the mice are expected to develop IE. During this period, the mice will undergo echocardiographic/PET-examination/in vivo hemodynamic examination and will then be sacrificed to collect heart valves and blood for further analysis. If successful, in establishing this model and reliably inducing IE, the next step will be to investigate other pathogens, such as Streptococcus and Enterococcus species and their interaction with valve cells. Further, the Cardiovascular research group in need for expertise in small animal cardiac imaging. The group have ongoing projects in cardiovascular aging, cardiometabolic and cardiorenal health. The candidate will have opportunity to participate and collaborate with other PIs. As this project is connected to a patient registry for infective endocarditis. Participation in epidemiological analysis and translational work is a possibility. Both a strong academic track record and a genuine interest in conducting research are essential to qualify for the position. The candidates, academic track record, and other application materials must demonstrate this. We will emphasize personal suitability in our assessment. Consequently, applicants are required to submit a vision statement detailing their motivation for joining the research project and group, how they intend to contribute to and enhance the research within the project and group, and their research ambitions. Contact For further information about the position, please contact Associate Professor Brage Håheim: email: Brage.haheim@uit.no phone:  99 54 53 23 Qualifications Required qualifications: A Norwegian doctoral degree (PhD) or an equivalent foreign doctoral degree in medicine, physiology, cardiology, infective disease or similar fields. A FELASA-approval for work with research animals. Documented experience in small animal research and experiments such as mice and rats. Candidates must be proficient small animal cardiac imaging and analysis (echocardiography/MRI/PET). Documented proficiency in both written and oral English. Applicants must have a documented knowledge of English which meets the requirements as stated in the Norwegian general university and college admission requirements. Nordic applicants can document their capabilities by attaching their high school diploma. Desired qualifications: Experience in animal research applications. Experience in invasive hemodynamic measurements (PV-loop catheters/thermodilution) as well as ex vivo hemodynamic measurements (isolated running hearts). Experience in molecular/cellular biology is not required, but an advantage. This includes experience with cardiac cell work (including cardiac/valve cell isolation), qPCR, histology, antibody-based techniques, confocal microscopy, small molecules/nanoparticles-based therapies, and live imaging. Working knowledge of Norwegian or a Scandinavian language is not a requirement, but an advantage. In the assessment the main emphasis will be attached to the submitted works. Emphasis shall also be attached to experience from popularization/dissemination and academic policy and administrative activity. During the assessment emphasis will be put on the candidate’s motivation, potential for research, and personal suitability for the position. We are looking for candidates who: Have good collaboration skills Have good communication and interaction with colleagues and students Wants to contribute to a good working environment We are seeking a creative and enthusiastic candidate with a high degree of self-motivation and possesses the ability to work in a structured manner. The candidate should also have high organizational skills and ability to perform clean work in the lab. At UiT we put emphasis on the quality, relevance and significance of the research work and not on where the work is published, in accordance with the principles of The San Francisco Declaration on Research Assessment (DORA). Inclusion and diversity UiT The Arctic University i Norway is working actively to promote equality, gender balance and diversity among employees and students, and to create an inclusive and safe working environment. We believe that inclusion and diversity is a strength, and we want employees with different competencies, professional experience, life experience and perspectives. If you have a disability, a gap in your

Position of PhD student Study of Microbial Ecology in Barrel-Aged Sour Beers

The Laboratory for Microbial Process Ecology and Control (PME&BIM, Centre for Microbial and Plant Genetics) belongs to Campus Group T and the Department of Microbial and Molecular Systems (M2S), and is also a member of the Leuven Institute for Beer Research (LIBR). The research group is internationally recognized for its expertise in microbial ecology and industrial microbiology. A key research line focuses on understanding spontaneous fermentations and maturation processes of food and alcoholic beverages. Unit website Project The aging of beer in wooden barrels, or the use of wood alternatives, has regained the interest of both the beer industry and consumers in recent years. The process can add various flavors or aroma components to the beer, originating from the wood itself or from the microbial community present in the wooden barrel or in the beer. In recent years, various studies have yielded important insights into the use of wood in the brewing process and the role of microorganisms therein. Within the research group, an in vitro system was developed that allows the process to be efficiently studied, manipulated, and monitored. In this way, the patterns and underlying mechanisms in the complex interaction between beer style, wood, and microorganisms can be uncovered. The in vitro system offers the possibility to study the influence of various variables, such as wood species, beer type, maturation temperature, composition of the microbial community, and interactions between them, on the quality of wood-matured beer. The formation and modification of flavor and aroma components will be monitored via metabolomics. The composition and dynamics of the microbial communities will be investigated using innovative RNA- and DNA-based techniques such as metagenetics, metagenomics, and metatranscriptomics. We are looking for a dynamic and motivated PhD student who will conduct innovative scientific research in the field of microbial ecology and applied bioinformatics. The use of various -omics tools means that the ideal candidate must not only possess excellent laboratory skills but also have a strong interest in delving into the field of applied bioinformatics to analyze the large amount of data generated. The research activities will result in a PhD at the Faculty of Industrial Engineering (KU Leuven) and have a clear, practically applicable purpose. Interim results will be published in scientific articles. The candidate also reports to interested companies and potential users of the results. The researcher will be part of an extensive international research team and will have the opportunity to collaborate with fellow researchers, students, companies, pilot-scale breweries, and other research institutions. Profile The candidate: Holds a Master’s degree in Industrial Engineering, Bioengineering, or Biology, or will soon obtain one. Has a strong interest in and knowledge of the research domains of microbiology, microbial ecology, and applied bioinformatics. Is communicative and willing to report on your research at (inter)national conferences, on social media, and through education. Is willing to supervise students on group projects, bachelor’s or master’s theses. – Is willing to provide support with teaching assignments. Is flexible, shows initiative, and is self-directed, but is also a team player. – Thinks in solutions, not problems. – Is collaborative and has an interdisciplinary mindset. Excellent knowledge of Dutch and English is required. – Experience in conducting experimental research is a plus. Experience with bioinformatics tools, programming, and/or a Linux environment is a plus, as is a good knowledge of brewing technology (and/or food technology). Offer Following an initial selection, suitable candidates will be invited for an interview scheduled for the second half of June 2026. Final recruitment will take place after these interviews. The selected candidate will be hired as a doctoral fellow with an initial contract of 1 year, extendable to 4 years upon positive evaluation. The workplace is the Laboratory for Microbial and Process Ecology and Management in Heverlee (Leuven). Interested? Complete contact persons in step 1 “Vacancy context” and regenerate this text. You can apply for this vacancy until 01/06/2026 via our online application system. KU Leuven aims to be an inclusive, respectful, and socially safe community. We embrace diversity among individuals and groups as an asset. Open dialogue and differences in perspective are essential in an ambitious research and teaching environment. In our pursuit of equal opportunities, we acknowledge the consequences of historical inequalities. We do not accept any form of discrimination based on, among other things, sex, gender identity and expression, sexual orientation, age, ethnic or national origin, skin colour, religious belief, neurodivergence, disability, health, or socio-economic status. If you have questions about accessibility or available support, we are happy to assist you at this email address . Application procedure Employment conditions Career opportunities Do you have a question about the online application procedure? Consult our frequently asked questions or send an email to solliciteren@kuleuven.be

Position of Doctoral Fellow in the Department of Basic and Applied Medical Sciences

ABOUT GHENT UNIVERSITY Ghent University is a world of its own. Employing more than 15,000 people, it is actively involved in education and research, management and administration, as well as technical and social service provision on a daily basis. It is one of the largest, most exciting employers in the area and offers great career opportunities. With its 11 faculties and more than 80 departments offering state-of-the-art study programmes grounded in research in a wide range of academic fields, Ghent University is a logical choice for its staff and students. YOUR TASKS The Gut-Liver ImmunoPharmacology Unit, in collaboration with the Hepatology Research Unit, is seeking a highly motivated PhD student for an innovative translational research project focused on liver diseases. In this project, you will play a crucial role in further unraveling the pathogenesis of chronic liver disease. The ultimate goal of the research is to obtain a doctoral degree. What You Will Do: •    Investigate the mechanisms underlying chronic liver diseases using human samples, animal models, and in vitro assays. •    Utilize a variety of techniques, including but not limited to: o    qRT-PCR o    ELISA o    Western blot o    Luminex o    Flow cytometry and FACS o    (Single cell) RNA-seq WHAT WE ARE LOOKING FOR You hold a master’s degree in Medicine, Pharmaceutical Sciences, Biomedical Sciences, or Veterinary Sciences. Excellent communication and writing skills in English. A driven and curious PhD student with a strong interest in immunology, hepatology and pharmacology. Experience with relevant techniques is a plus, but motivation and eagerness to learn are equally important. WHAT WE CAN OFFER YOU We offer a full-time position as a doctoral fellow, consisting of an initial period of 12 months, which – after a positive evaluation, will be extended to a total maximum of 48 months. Your contract will start on 10/01/2026 at the earliest. The fellowship amount is 100% of the net salary of an AAP member in equal family circumstances. The individual fellowship amount is determined by Team Personnel Administration based on family status and seniority. A grant that meets the conditions and criteria of the regulations for doctoral fellowships is considered free of personal income tax. Click here for more information about our salary scales All Ghent University staff members enjoy a number of benefits, such as a wide range of training and education opportunities, 36 days of holiday leave (on an annual basis for a full-time job) supplemented by annual fixed bridge days, bicycle allowance and eco vouchers. Click here for a complete overview of all the staff benefits. INTERESTED? Send your cv, copy of your diploma (if already in your possession) and a motivation letter to lindsey.devisscher@ugent.be.We do not accept late applications. As Ghent University maintains an equal opportunities and diversity policy, everyone is encouraged to apply for this position. For more information about this vacancy, please contact prof. Lindsey Devisscher (lindsey.devisscher@ugent.be, +32(0)9/332 56 65)

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