PhD Positions – Time to die: Cell death as a tool to improve bioprocesses
Your job Do you have a passion for understanding cellular mechanisms and microbial physiology, and translating this to bioprocesses? Are you driven by the challenge of pushing biotechnological innovation to the next level? This PhD position might be for you! In this specific project, you will select specific regulated cell death pathways to be used as cell death switch. By coupling controllable cell death induction with productivity your aim is to increase process efficiency, without compromising product quality. This knowledge will be used to improve the bioproduction of chemicals, including pigments. Understanding and Postponing Yeast Cell Death (UPsYDe) to improve production is a doctoral network of 13 PhD candidates funded by Horizon Europe: Marie Skłodowska-Curie Actions. UPsYDe consists of seven host institutes (Wageningen Universiteit, Universidade do Minho, Danmarks Tekniske Universitet, Austrian Centre for Industrial Biotechnology, Imperial College London, Università degli Studi di Milano Bicocca and Chalmers Tekniska Högskola) together with fifteen associated partners, ranging from start-up to large biotechnology companies and renowned research institutes. This consortium aims to understand how yeast cells die under industrial process conditions and to use this knowledge to improve biomanufacturing. Different yeast species play a vital role in industrial biotechnology for the sustainable production of a wide range of products, for example ethanol as a biofuel or protein ingredients for alternatives to animal-based products. However, also the lifespan of these yeast cells is finite, and their death negatively impacts processes: for example, process duration or product quality. Fundamental research has revealed that yeast cells can die in different ways, i.e., different regulated cell death programs are present and (genetic) factors involved in these processes are being uncovered. However, in the context of industrial processes, regulated cell death has received little attention. Therefore, we will integrate fundamental cell and molecular biology, systems and synthetic biology with bioprocess and metabolic engineering to improve both our understanding of these cell death processes as well as the survival and performance of different yeast species in industrial bioprocesses. Each of 13 doctoral projects will focus on a different aspect, more detailed information can be found on upsyde.eu, but in strong synergy with the other projects. At Wageningen we are looking for two PhD candidates. Each PhD will receive a strongly interdisciplinary training alongside performing exciting research. This includes secondments at two or more partners in the consortium. Your duties and responsibilities include: develop synthetic biology tools to induce specific RCD pathways in yeast; construct genetic circuits to couple productivity with specific induction; investigate the link between cell death mode and product quality; improve productivity of yeast bioprocesses by specific and “on demand” RCD activation; work together with the other 12 DCs to integrate and translate knowledge to other yeast species. You will work here The research is embedded within the chair Bioprocess Engineering, and is part of the Microbial Biotechnology team, which is led by Prof. Dr. Ruud Weusthuis and Dr. Mark Bisschops. You will be supervised by Dr. Mark Bisschops. Within the project, you will collaborate with the other consortium members of UPsYDE and especially the Laboratory of Systems and Synthetic Biology (SSB) at Wageningen University, the laboratory of Prof. Rodrigo Ledesma-Amaro at Imperial College (London, United Kingdom) and the company Octarine Bio in Denmark. Your qualities You have a passion for understanding cellular mechanisms and microbial physiology and translating this to bioprocesses, and have high standards for working accurately. You have the flexibility to work in a team within a well-equipped laboratory and communicate results to experts of different, but related fields. You also possess: a successfully completed MSc degree in biotechnology or related field; the ability to work independently in the lab; a strong desire and ability to quickly master new (laboratory) techniques to effectively tackle complex, multidisciplinary scientific queries; experience with microbial cultures, molecular biology and, ideally, bioreactor cultivations; experience with fluorescence microscopy, omics analyses and yeast are a pre. For this position your command of the English language is expected to be at C1 level. Sometimes it is necessary to submit an internationally recognised Certificate of Proficiency in the English Language. More information can be found here. Because this concerns a Marie-Sklodowska-Curie Action funded position, additional elegibility criteria apply: not be in possession of a doctoral degree at the date of the recruitment; comply with the mobility rule: no residence or main activity (work, studies, etc.) in the country of the recruiting beneficiary for more than 12 months in the 36 months before their recruitment date), this includes the country of the institution for which the main activity was performed. We offer Wageningen University & Research offers excellent terms of employment. A few highlights from our Collective Labour Agreement include: partially paid parental leave; working hours that can be discussed and arranged so that they allow for the best possible work-life balance; there is a strong focus on vitality and you can make use of the sports facilities available on campus for a small fee; a fixed year-end bonus of 8.3%; excellent pension scheme. In addition to these first-rate employee benefits, you will receive a fully funded PhD position and you will be offered a course program tailored to your needs and the research team. The gross salary for the first year is € 3.059,- per month rising to € 3.881,- in the fourth year in according to the Collective Labour Agreements for Dutch Universities (CAO-NU) (scale P). This is based on a full-time working week of 38 hours. We offer a temporary contract for 18 months which will be extended for the duration of the project if you perform well. We encourage development and internal mobility within our organisation. Our recruitment and selection policy sets out the conditions that apply specifically to you as a (former) employee. If you have any questions, we are happy to help. You will work on the greenest and most innovative campus in the Netherlands, in an international and open working environment. Coming from abroad Wageningen University & Research is the university and research centre for life sciences. The themes we deal with are relevant to everyone around the world and Wageningen,

