Optimisation of integrated delivery systems for probiotic yeast Saccharomyces boulardii to enhance its survival and applications in functional nutrition and health

Summary

Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer health benefits on the host.

Among various probiotics, including bacteria and yeast cultures, Saccharomyces boulardii is the only yeast widely recognised for its beneficial effects on gastrointestinal health and immune function in humans [1-2].

There are numerous studies in the field of probiotic bacteria, such as strains of lactic acid bacteria; however, fewer attempts have been made for the stability modifications of probiotic yeast S. boulardii for its beneficial applications in functional nutrition products for health.

This project aims to develop an integrated, multi-stage strategy to improve the tolerance and survival of probiotic yeast for its effective and stable application in nutrition formulation and health products [3].

Objectives

1. To isolate and screen naturally occurring heat-tolerant strains of probiotic yeast S. boulardii from diverse sources.
2. To apply controlled adaptive protocols by incubating selected strains at elevated, sub-lethal temperatures to enhance their intrinsic thermal tolerance and robustness.
3. To optimise the stability of yeast cells by applying Immobilisation and encapsulation techniques.
4. To encapsulate the adapted S. boulardii strains using multi-component matrices (e.g., Alginate, Chitosan, and Starch derivatives) via advanced techniques such as Extrusion and Emulsification to maximise cellular protection.
5. Preparation of multi-component encapsulation matrices. Utilisation of Extrusion and Emulsification techniques for cell entrapment. Optimisation of bead size, cell loading, and encapsulation efficiency.
6. To evaluate various drying methods (Spray-Drying and Freeze-Drying) to determine the optimal process for maximising long-term stability and shelf-life of the encapsulated product.
7. To test the survival rates of the optimised, encapsulated probiotic under simulated gastrointestinal conditions to confirm their industrial applicability.

Expected Outcomes

• Provision of an optimised, encapsulated probiotic model ready for use in future in vivo or animal studies to investigate colonic microbiota modulation.
• A validated protocol for maintaining the functionality and stability of S. boulardii under severe thermal and acidic conditions.
• Development of a highly protective and industrially viable encapsulation system suitable for the functional food market.

Essential criteria

Applicants should hold, or expect to obtain, a First or Upper Second Class Honours Degree in a subject relevant to the proposed area of study.

We may also consider applications from those who hold equivalent qualifications, for example, a Lower Second Class Honours Degree plus a Master’s Degree with Distinction.

In exceptional circumstances, the University may consider a portfolio of evidence from applicants who have appropriate professional experience which is equivalent to the learning outcomes of an Honours degree in lieu of academic qualifications.

• Sound understanding of subject area as evidenced by a comprehensive research proposal
• A comprehensive and articulate personal statement

Desirable Criteria

If the University receives a large number of applicants for the project, the following desirable criteria may be applied to shortlist applicants for interview.

• First Class Honours (1st) Degree
• Completion of Masters at a level equivalent to commendation or distinction at Ulster
• Practice-based research experience and/or dissemination
• Experience using research methods or other approaches relevant to the subject domain
• Work experience relevant to the proposed project
• Publications record appropriate to career stage
• Experience of presentation of research findings

Equal Opportunities

The University is an equal opportunities employer and welcomes applicants from all sections of the community, particularly from those with disabilities.

Appointment will be made on merit.

Funding and eligibility

NOTE – This is a self funded research project and applicants will be required to provide evidence of funds to support their tuition fees and living expenses.

Applicants should hold, or expect to obtain, a First or Upper Second Class Honours Degree in a subject relevant to the proposed area of study.